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Image Search Results
Journal: Cell reports
Article Title: Redox-sensitive E2 Rad6 controls cellular response to oxidative stress via K63-linked ubiquitination of ribosomes
doi: 10.1016/j.celrep.2022.110860
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Virus, Recombinant, Ubiquitin Proteomics, Protease Inhibitor, Mass Spectrometry, Mutagenesis, Multiplex sample analysis, Expressing, Software
Journal: Physical biology
Article Title: Facile single-molecule pull-down assay for analysis of endogenous proteins
doi: 10.1088/1478-3975/ab0792
Figure Lengend Snippet: Direct adhesion of proteins to DDS surface. (a) Schematic of direct attachment of proteins. (b) Average number of molecules per field-of-view comparing direct attachment of 10 nM tubulin-TMR to DDS sandwich SiMPull using 3 nM tubulin-TMR. (c) Representative images of direct adhesion of EGFP showing the loss of EGFP fluorescence with EGFP shown in green and F(ab)-A647 shown in red. Scale bars, 10 μm.
Article Snippet: For experiments where a protein was adsorbed directly onto the
Techniques: Fluorescence
Journal: Physical biology
Article Title: Facile single-molecule pull-down assay for analysis of endogenous proteins
doi: 10.1088/1478-3975/ab0792
Figure Lengend Snippet: Endogenous α-tubulin DDS SiMPull using mammalian cell lysates. (a) The average number of α-tubulin molecules per field-of-view for traditional DDS SiMPull, pre-incubation of the detection antibodies in solution, and nonspecific binding. Error bars show the standard deviation from the mean. Intensity distributions and representative images for (b) DDS sandwich SiMPull, (c) pre-incubation of 1°AB and F(ab)-A647, and (d) pre-incubation of lysate, 1°AB, and F(ab)-A647. Scale bars, 10 μm.
Article Snippet: For experiments where a protein was adsorbed directly onto the
Techniques: Incubation, Binding Assay, Standard Deviation